Respon Pertumbuhan dan Hasil Tanaman Bayam terhadap Aplikasi Ecoenzyme Berbahan Dasar Limbah Pertanian Lokal Banyuwangi

https://doi.org/10.47637/agrimals.v6i1.2372
Published: May 30, 2026

Abstract:

Ecoenzyme (EE) is a fermented liquid derived from organic fruit and vegetable waste that has the potential to enhance plant growth and support sustainable agriculture. This study aimed to evaluate the effect of different organic waste-based Ecoenzyme formulations on the growth of spinach plants. The experiment was conducted using a pot experiment arranged in a Completely Randomized Design (CRD) consisting of four treatments: P1 (mustard greens + mangosteen peel), P2 (mustard greens + orange peel), P3 (mustard greens + dragon fruit peel), and P4 (mustard greens only). The observed growth parameters included plant height, leaf number, fresh weight, and total chlorophyll content. The results showed that Ecoenzyme application significantly affected plant height and fresh weight, while the number of leaves did not differ significantly among treatments. These findings indicate that Ecoenzyme produced from a combination of vegetable and fruit organic wastes has the potential to be utilized as an alternative liquid organic fertilizer to support spinach cultivation.

Keywords:
1. Amaranthus tricolor L.
2. ecoenzyme
3. efisiensi
4. fermentasi
5. limbah agroindustri
Authors:
1 . Ari Istanti
Politeknik Negeri Banyuwangi
2 . Aldy Bahaduri Indraloka
Politeknik Negeri Banyuwangi
3 . Sari Wiji Utami
Politeknik Negeri Banyuwangi
4 . Riska Ardianti
Politeknik Negeri Banyuwangi
5 . Selvi Nur Dinah
Politeknik Negeri Banyuwangi
6 . Veronica Nafa Urridha
Politeknik Negeri Banyuwangi
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How to Cite
Istanti, A., Aldy Bahaduri Indraloka, Sari Wiji Utami, Riska Ardianti, Selvi Nur Dinah, & Urridha, V. N. (2026). Respon Pertumbuhan dan Hasil Tanaman Bayam terhadap Aplikasi Ecoenzyme Berbahan Dasar Limbah Pertanian Lokal Banyuwangi. Journal of Agriculture and Animal Science, 6(1), 118–128. https://doi.org/10.47637/agrimals.v6i1.2372
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Issue & Section
Vol. 6 No. 1 (2026): Volume 6 Nomor 1 Tahun 2026
Articles
References

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    Arun, C., & Sivashanmugam, P. (2015). Solubilization of waste activated sludge using a garbage enzyme produced from different pre-consumer organic waste. RSC Advances, 5(63), 51421–51427. https://doi.org/10.1039/C5RA07959D

    Badan Pusat Statistik. (2021). Kabupaten Banyuwangi Dalam Angka 2021. https://banyuwangikab.bps.go.id/publication/2021/02/26/92c9d6985269031f62f278b4/kabupa%0Aten-banyuwangi-dalam-angka-2021.html

    Croft, H., Chen, J. M., Luo, X., Bartlett, P., Chen, B., & Staebler, R. M. (2017). Leaf chlorophyll content as a proxy for leaf photosynthetic capacity. Global Change Biology, 23(9), 3513–3524.https://doi.org/10.1111/GCB.13599;JOURNAL:JOURNAL:13652486;WGROUP:STRING:PUBLICATION

    Evans, J. R. (1989). Photosynthesis and nitrogen relationships in leaves of C3 plants. Oecologia, 78(1), 9–19. https://doi.org/10.1007/BF00377192/METRICS

    Gustia, H., Wulandari, Y. A., Rosdiana, & Putri, D. (2025). Optimizing Eco-enzyme Dosage on Kale (Brassica oleracea) Growth and Production. JURNAL AGRONOMI TANAMAN TROPIKA (JUATIKA), 7(1), 164-169–164 – 169. https://doi.org/10.36378/JUATIKA.V7I1.3985

    Gutierrez-Orozco, F., & Failla, M. L. (2013). Biological Activities and Bioavailability of Mangosteen Xanthones: A Critical Review of the Current Evidence. Nutrients 2013, Vol. 5, Pages 3163-3183, 5(8), 3163–3183. https://doi.org/10.3390/NU5083163

    Huarancca Reyes, T., Scartazza, A., Pompeiano, A., Ciurli, A., Lu, Y., Guglielminetti, L., & Yamaguchi, J. (2018). Nitrate Reductase Modulation in Response to Changes in C/N Balance and Nitrogen Source in Arabidopsis. Plant and Cell Physiology, 59(6), 1248–1254. https://doi.org/10.1093/PCP/PCY065

    Istanti, A., Indraloka, A. B., & Utami, S. W. (2023). Karakteristik Pupuk Cair Eco-Enzyme Berbahan Dasar Limbah Sayur Dan Buah Terhadap Kandungan Nutrisi Dan Bahan Organik. Agriprima.7 (1) : 79-85.

    Javed, T., Indu, I., Singhal, R. K., Shabbir, R., Shah, A. N., Kumar, P., Jinger, D., Dharmappa, P. M., Shad, M. A., Saha, D., Anuragi, H., Adamski, R., & Siuta, D. (2022). Recent Advances in Agronomic and Physio-Molecular Approaches for Improving Nitrogen Use Efficiency in Crop Plants. Frontiers in Plant Science, 13, 877544. https://doi.org/10.3389/FPLS.2022.877544/FULL

    Jung, H. A., Su, B. N., Keller, W. J., Mehta, R. G., & Kinghorn, A. D. (2006). Antioxidant Xanthones from the Pericarp of Garcinia mangostana (Mangosteen). Journal of Agricultural and Food Chemistry, 54(6), 2077–2082. https://doi.org/10.1021/JF052649Z

    Kurnia Illahi, A., Kurniasih, D., Andam Sari, D., Karmaita, Y., Studi Teknologi Produksi Tanaman Pangan, P., Budi Daya Tanaman, J., Pertanian Negeri Payakumbuh Jl Raya Negara, P. K., & Pati, T. (2023). Analisis Kualitas Eco Enzym Dari Berbagai Bahan Dasar Kulit Buah Untuk Pertanian Berkelanjutan. Agrisaintifika: Jurnal Ilmu-Ilmu Pertanian, 7(1), 76–81. https://doi.org/10.32585/AGS.V7I1.3675

    Lim, S. L., Lee, L. H., & Wu, T. Y. (2016). Sustainability of using composting and vermicomposting technologies for organic solid waste biotransformation: recent overview, greenhouse gases emissions and economic analysis. Journal of Cleaner Production, 111, 262–278. https://doi.org/10.1016/J.JCLEPRO.2015.08.083

    Listiana, I., Ayuni, T., Asih Saputri, D., Amelia, I., Studi Biologi, P., Tarbiyah dan Keguruan, F., Raden Intan Lampung, U., & Studi Pendidikan Biologi, P. (2024). Utilization of Eco-Enzyme on Land Spinach (Ipomoea reptans Poir.) with Wick Hydroponic System. Biospecies, 17(2), 22–28. https://doi.org/10.22437/biospecies.v17i2.35787

    Meisy Evis Putri, R., Fevria, R., Departemen Biologi, V., Matematika dan Ilmu Pengetahuan Alam, F., Negeri Padang Jl Hamka, U., Padang, K., & Barat, S. (2023). Pengaruh Ecoenzyme Teknologi Nano Terhadap Pertumbuhan Pakcoy (Brassica rapa L.) yang Dibudidayakan Secara Hidroponik. Produksi Tanaman, 11(6), 349–358. https://doi.org/10.21776/UB.PROTAN.2023.011.06.01

    Mubarok, S., Ananda, S. D., Farida, F., Fadilah, A., & Sudirja, R. (2021). Evaluasi tiga sistem budidaya di lahan sempit pada budidaya dua kultivar bayam di Kota Bekasi. Kultivasi, 20(2). https://doi.org/10.24198/KULTIVASI.V20I2.32022

    Novianto. (2022). Response Of Liquid Organic Fertilizer Eco Enzyme (EE) On Growth And Production Of Shallot (Allium Ascalonicum. L). JURNAL AGRONOMI TANAMAN TROPIKA (JUATIKA), 4(1), 147–154. https://doi.org/10.36378/JUATIKA.V4I1.1782

    Nurfadilah, F., Surtikanti, H. K., & Nilawati, T. S. (2024). Pertumbuhan tanaman bayam horenzo (Spinacia orelacea L.) dengan pemberian nutrisi menggunakan ekoenzim. Holistic: Journal of Tropical Agriculture Sciences, 1(2). https://doi.org/10.61511/HJTAS.V1I2.2024.333

    Rasit, N., Fern, L. H., & Ghani, A. W. A. K. (2019). Production and Characterization of Eco Enzyme Produced From Tomato and Orange Wastes and Its Influence On The Aquaculture Sludge. International Journal of Civil Engineering and Technology, 10(03), 967–980.

    Rasit, N., Hwe Fern, L., & Ab Karim Ghani, W. A. W. (2019). Production and Characterization of Eco Enzyme Produced from Tomato and Orange Wastes and Its Influence on the Aquaculture Sludge. https://papers.ssrn.com/abstract=3456453

    Paramudita, S. , K. B., Ilmiasari, Y., Harini, N. V. A., & Novrimansyah, E. A. (2025). Pengaruh pemberian POC Air Cucian Beras Pada Hasil Produksi Tanaman Selada (Lactuca sativa L.). Journal of Agriculture and Animal Science, 5(1), 11–20. https://doi.org/10.47637/AGRIMALS.V5I1.1449

    Setiawati, P. L., Sumardi, S., Irawan, B., Nurcahyani, E., & Agustrina, R. (2023). Penentuan Karakteristik dan Efektivitas Eco Enzyme Berbahan Dasar Limbah Organik yang Berbeda sebagai Pengawet Buah Tomat (Solanum esculentum MILL.): Determination of The Characteristics And Effectiveness of Eco enzyme Based on Different Organic Waste Fo…. Biospecies, 16(1), 80–87. https://doi.org/10.22437/biospecies.v16i1.24110

    Sinaga, W. S., Limeranto, D. M., Pangala, E. L. B., & Madyaningrana, K. (2023). Efek Pemberian Pupuk Organik Cair Berbasis Kulit Buah (Eco Enzyme) terhadap Pertumbuhan Tanaman Pakcoy (Brassica chinensis L.). Pro-Life, 10(2), 839–852. https://doi.org/10.33541/PRO-LIFE.V10I2.4801

    Suri, A. M. (2025). Journal of Agriculture and Animal Science Respons Pakcoy (Brassica rapa) Akibat Teknik Pemupukan Terhadap Karakter Fisiologis Dan Hasil Panen. https://doi.org/10.47637/agrimals.v5i2.1926

    Tang, F. E., & Tong, C. W. (2011). A Study of the Garbage Enzyme’s Effects in Domestic Wastewater. International Journal of Environmental and Ecological Engineering, 5(12), 887–892. https://doi.org/10.5281/ZENODO.1332982

  1. Anas, M., Liao, F., Verma, K. K., Sarwar, M. A., Mahmood, A., Chen, Z. L., Li, Q., Zeng, X. P., Liu, Y., & Li, Y. R. (2020). Fate of nitrogen in agriculture and environment: agronomic, eco-physiological and molecular approaches to improve nitrogen use efficiency. Biological Research 2020 53:1, 53(1), 47-. https://doi.org/10.1186/S40659-020-00312-4
  2. Arun, C., & Sivashanmugam, P. (2015). Solubilization of waste activated sludge using a garbage enzyme produced from different pre-consumer organic waste. RSC Advances, 5(63), 51421–51427. https://doi.org/10.1039/C5RA07959D
  3. Badan Pusat Statistik. (2021). Kabupaten Banyuwangi Dalam Angka 2021. https://banyuwangikab.bps.go.id/publication/2021/02/26/92c9d6985269031f62f278b4/kabupa%0Aten-banyuwangi-dalam-angka-2021.html
  4. Croft, H., Chen, J. M., Luo, X., Bartlett, P., Chen, B., & Staebler, R. M. (2017). Leaf chlorophyll content as a proxy for leaf photosynthetic capacity. Global Change Biology, 23(9), 3513–3524.https://doi.org/10.1111/GCB.13599;JOURNAL:JOURNAL:13652486;WGROUP:STRING:PUBLICATION
  5. Evans, J. R. (1989). Photosynthesis and nitrogen relationships in leaves of C3 plants. Oecologia, 78(1), 9–19. https://doi.org/10.1007/BF00377192/METRICS
  6. Gustia, H., Wulandari, Y. A., Rosdiana, & Putri, D. (2025). Optimizing Eco-enzyme Dosage on Kale (Brassica oleracea) Growth and Production. JURNAL AGRONOMI TANAMAN TROPIKA (JUATIKA), 7(1), 164-169–164 – 169. https://doi.org/10.36378/JUATIKA.V7I1.3985
  7. Gutierrez-Orozco, F., & Failla, M. L. (2013). Biological Activities and Bioavailability of Mangosteen Xanthones: A Critical Review of the Current Evidence. Nutrients 2013, Vol. 5, Pages 3163-3183, 5(8), 3163–3183. https://doi.org/10.3390/NU5083163
  8. Huarancca Reyes, T., Scartazza, A., Pompeiano, A., Ciurli, A., Lu, Y., Guglielminetti, L., & Yamaguchi, J. (2018). Nitrate Reductase Modulation in Response to Changes in C/N Balance and Nitrogen Source in Arabidopsis. Plant and Cell Physiology, 59(6), 1248–1254. https://doi.org/10.1093/PCP/PCY065
  9. Istanti, A., Indraloka, A. B., & Utami, S. W. (2023). Karakteristik Pupuk Cair Eco-Enzyme Berbahan Dasar Limbah Sayur Dan Buah Terhadap Kandungan Nutrisi Dan Bahan Organik. Agriprima.7 (1) : 79-85.
  10. Javed, T., Indu, I., Singhal, R. K., Shabbir, R., Shah, A. N., Kumar, P., Jinger, D., Dharmappa, P. M., Shad, M. A., Saha, D., Anuragi, H., Adamski, R., & Siuta, D. (2022). Recent Advances in Agronomic and Physio-Molecular Approaches for Improving Nitrogen Use Efficiency in Crop Plants. Frontiers in Plant Science, 13, 877544. https://doi.org/10.3389/FPLS.2022.877544/FULL
  11. Jung, H. A., Su, B. N., Keller, W. J., Mehta, R. G., & Kinghorn, A. D. (2006). Antioxidant Xanthones from the Pericarp of Garcinia mangostana (Mangosteen). Journal of Agricultural and Food Chemistry, 54(6), 2077–2082. https://doi.org/10.1021/JF052649Z
  12. Kurnia Illahi, A., Kurniasih, D., Andam Sari, D., Karmaita, Y., Studi Teknologi Produksi Tanaman Pangan, P., Budi Daya Tanaman, J., Pertanian Negeri Payakumbuh Jl Raya Negara, P. K., & Pati, T. (2023). Analisis Kualitas Eco Enzym Dari Berbagai Bahan Dasar Kulit Buah Untuk Pertanian Berkelanjutan. Agrisaintifika: Jurnal Ilmu-Ilmu Pertanian, 7(1), 76–81. https://doi.org/10.32585/AGS.V7I1.3675
  13. Lim, S. L., Lee, L. H., & Wu, T. Y. (2016). Sustainability of using composting and vermicomposting technologies for organic solid waste biotransformation: recent overview, greenhouse gases emissions and economic analysis. Journal of Cleaner Production, 111, 262–278. https://doi.org/10.1016/J.JCLEPRO.2015.08.083
  14. Listiana, I., Ayuni, T., Asih Saputri, D., Amelia, I., Studi Biologi, P., Tarbiyah dan Keguruan, F., Raden Intan Lampung, U., & Studi Pendidikan Biologi, P. (2024). Utilization of Eco-Enzyme on Land Spinach (Ipomoea reptans Poir.) with Wick Hydroponic System. Biospecies, 17(2), 22–28. https://doi.org/10.22437/biospecies.v17i2.35787
  15. Meisy Evis Putri, R., Fevria, R., Departemen Biologi, V., Matematika dan Ilmu Pengetahuan Alam, F., Negeri Padang Jl Hamka, U., Padang, K., & Barat, S. (2023). Pengaruh Ecoenzyme Teknologi Nano Terhadap Pertumbuhan Pakcoy (Brassica rapa L.) yang Dibudidayakan Secara Hidroponik. Produksi Tanaman, 11(6), 349–358. https://doi.org/10.21776/UB.PROTAN.2023.011.06.01
  16. Mubarok, S., Ananda, S. D., Farida, F., Fadilah, A., & Sudirja, R. (2021). Evaluasi tiga sistem budidaya di lahan sempit pada budidaya dua kultivar bayam di Kota Bekasi. Kultivasi, 20(2). https://doi.org/10.24198/KULTIVASI.V20I2.32022
  17. Novianto. (2022). Response Of Liquid Organic Fertilizer Eco Enzyme (EE) On Growth And Production Of Shallot (Allium Ascalonicum. L). JURNAL AGRONOMI TANAMAN TROPIKA (JUATIKA), 4(1), 147–154. https://doi.org/10.36378/JUATIKA.V4I1.1782
  18. Nurfadilah, F., Surtikanti, H. K., & Nilawati, T. S. (2024). Pertumbuhan tanaman bayam horenzo (Spinacia orelacea L.) dengan pemberian nutrisi menggunakan ekoenzim. Holistic: Journal of Tropical Agriculture Sciences, 1(2). https://doi.org/10.61511/HJTAS.V1I2.2024.333
  19. Rasit, N., Fern, L. H., & Ghani, A. W. A. K. (2019). Production and Characterization of Eco Enzyme Produced From Tomato and Orange Wastes and Its Influence On The Aquaculture Sludge. International Journal of Civil Engineering and Technology, 10(03), 967–980.
  20. Rasit, N., Hwe Fern, L., & Ab Karim Ghani, W. A. W. (2019). Production and Characterization of Eco Enzyme Produced from Tomato and Orange Wastes and Its Influence on the Aquaculture Sludge. https://papers.ssrn.com/abstract=3456453
  21. Paramudita, S. , K. B., Ilmiasari, Y., Harini, N. V. A., & Novrimansyah, E. A. (2025). Pengaruh pemberian POC Air Cucian Beras Pada Hasil Produksi Tanaman Selada (Lactuca sativa L.). Journal of Agriculture and Animal Science, 5(1), 11–20. https://doi.org/10.47637/AGRIMALS.V5I1.1449
  22. Setiawati, P. L., Sumardi, S., Irawan, B., Nurcahyani, E., & Agustrina, R. (2023). Penentuan Karakteristik dan Efektivitas Eco Enzyme Berbahan Dasar Limbah Organik yang Berbeda sebagai Pengawet Buah Tomat (Solanum esculentum MILL.): Determination of The Characteristics And Effectiveness of Eco enzyme Based on Different Organic Waste Fo…. Biospecies, 16(1), 80–87. https://doi.org/10.22437/biospecies.v16i1.24110
  23. Sinaga, W. S., Limeranto, D. M., Pangala, E. L. B., & Madyaningrana, K. (2023). Efek Pemberian Pupuk Organik Cair Berbasis Kulit Buah (Eco Enzyme) terhadap Pertumbuhan Tanaman Pakcoy (Brassica chinensis L.). Pro-Life, 10(2), 839–852. https://doi.org/10.33541/PRO-LIFE.V10I2.4801
  24. Suri, A. M. (2025). Journal of Agriculture and Animal Science Respons Pakcoy (Brassica rapa) Akibat Teknik Pemupukan Terhadap Karakter Fisiologis Dan Hasil Panen. https://doi.org/10.47637/agrimals.v5i2.1926
  25. Tang, F. E., & Tong, C. W. (2011). A Study of the Garbage Enzyme’s Effects in Domestic Wastewater. International Journal of Environmental and Ecological Engineering, 5(12), 887–892. https://doi.org/10.5281/ZENODO.1332982